Thrombin from bovine plasma；Factor IIa CAS 9002-04-4
Item No.： E0085
Store at： -20℃
English name：Thrombin from bovine plasma；Factor IIa
Other names: Thromboplastin; Blood coagulation factor 3; Protokinase; Coagulation factor.
Molecular weight：335800
Grade：BR
Source: Bovine plasma
Vitality：40～300 NIH units/mg protein
Protein：40～60%
Physicochemical properties: White or off white amorphous powder or solid. Soluble in water, insoluble in organic solvents.The dry powder is very stable at 2 ~ 8 ℃ and has a validity period of 3A; The aqueous solution was inactivated within 8h at room temperature. In case of heat, dilute acid, alkali and metal, the activity decreases. Prothrombin is isolated from animal plasma and activated by coagulation activating enzyme and calcium chloride. It is a kind of proteolytic enzyme. Under the activation of thrombin, soluble fibrinogen in plasma is transformed into insoluble fibrin network structure to coagulate blood.Fibrin is originally composed of six peptide chains. The composition of thrombin is to cut off a specific peptide bond (- Arg -Gly) from the N-end of the four peptide chains and release two a peptides (19 peptides) and two B peptides (21 peptides). The other two peptide chains with cool acid at the N-end remain unchanged.After the removal of A peptide and B peptide, the negative charge of protein molecules is reduced, and the linear polymerization and lateral polymerization of fibrin molecules are promoted, so as to form a fibrous egg with network structure.
Purpose: Biochemical research. An enzyme that converts fibrinogen to fibrin.
