Papain CAS 9001-73-4
Item No.： E0009
Store at： RT
English Name: Papain
Other names: Papain; Papaya prion enzyme; Papaya enzyme; Papain
Grade: BR
Molecular weight: 21000 ~ 23000
Vitality: ≥ 800u / mg
Moisture: ≤ 8.0%
Ash content: ≤ 12%
Lead (calculated by Pb): ≤ 2.0mg/kg
Arsenic (as): ≤ 2.0mg/kg
Optimum temperature: 65 ℃
Optimum pH: 6.0 ~ 7.0
Physicochemical properties : white to light yellow powder. An enzyme that hydrolyzes polypeptides, amides, and esters. The molecule consists of a folded polypeptide bond of 212 residues. Slightly hygroscopic. Sensitive to light. Not completely soluble in water and glycerol, almost insoluble in most organic solvents. Enzyme reaction: cleave Arg -, Lys -, phe -, X amino acids. The model substrates were benzoyl arginine ethyl ester (baee) and n-benzoyl-l-arginine p-nitroaniline (n- α- Bz-l-arg-4-na), benzyloxycarbonyl valeryl glycyl arginine p-nitroaniline (chromozym try). Activators include cysteine (Cys), glutathione (GSH), EDTA, reductant and cyanide. Inhibitors include mercury (Hg2 +), other heavy metals, iodoacetic acid, n-p-yl maleimide (NEM), phenylmethylsulfonyl fluoride (PMSF), n-p-toluenesulfonyl-l-phenylalanine chloromethyl ketone (TPCK) , p-toluenesulfonyl-l-lysine chloromethyl ketone (TLCK)
Purpose: biochemical research.
