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EcoRI endonuclease

CAS No.:
Item No.: E0228
Store at: -20℃

Overview: this restriction enzyme recognizes the cleavage site of 5 '- G ↑ aattc-3' and has the best activity in EcoRI buffer.

Features: all endonucleases have 100% activity in the optimal buffer and unified buffer; The buffer is 100% compatible with downstream experimental applications.

Application: enzyme digestion

Instructions for use: high enzyme concentration and the use of non optimal buffer may lead to star activity. Do not incubate for a long time when using BSA. In order to obtain 100% activity, BSA should be added to the 1x reaction mixture to a final concentration of 100 μ g/ ml.

Component:5KU/EcoRI,10X buffer EcoR I,10X Single buffer


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