Sephacryl S-400 High resolution CAS 65546-95-4
Item No.： I0092
Store at： 2～8°C
English name：Sephacryl S-400 High resolution
Grade：BR
Useful fractionation range(MW)：globular proteins 2×104～8×106；dextrans 1×104～2×106
DNA exlusion limit(base pairs)：271
Bead form:Spherical,diameter 25-75um in wet form
Bead structure：Allyl dextran and N,N-methylene bisacrylamide
pH stability：long term 3～11,short term 2～13
Chemical stability：Stable to all commonly used fuffers:0.2M NaOH；0.1M HC1；1M acetic acid；8M urea；6M guanidine HC1；1% SDS；2M NaC1；24% ethanol；30% propanol；30% acetonitrile(tested at 40℃ for 7 days)
Physical stability：Negligible volume variation due to changes in pH or ionic strength
Physicochemical properties: 20% ethanol, colloid at the bottom.
Purpose: Biochemical research.For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans
