Octyl agarose gel/CL-4B/ Octyl-Sepharose CL-4B
Item No.： I0365
Store at： 4℃
Physicochemical properties: white microsphere, gelatinous, preserved in 20% alcohol solution.
Gel type: hydrophobic chromatography packing
Structural composition: 4% cross-linked agarose
Particle size: 45-165 μ m
Working pH value: 2-14 (short time); 3-12 (long time)
Operating temperature: 4-40 ℃
Ligand density: 35 μ mol/mL
Application: hydrophobic chromatography medium, suitable for the separation and purification of biological macromolecules and vaccines.
Purpose: octyl agarose gel CL-4B is a hydrophobic medium that can be used to purify and separate target products by using octyl bond on agarose gel CL-4B.
Preservation: the product shall be stored in a sealed place at 4 ℃ ~ 25 ℃ (the preservation solution is 20% ethanol), ventilated, dry and clean, and shall not be frozen. The used column is stored at 4 ℃ (20% ethanol).
matters needing attention:
(1) Before loading the sample, the sample must be filtered through the membrane and the pigment must be removed, otherwise the impurities and pigment will be adsorbed on the filler and affect the normal use of the filler. All buffers need to be filtered with a 0.45um filter.
(2) In the process of use, avoid using strong acids and bases with high concentration, and the concentration of acids and bases shall be less than 0.1 mol. Alkali slows the flow rate.
(3) Different samples have different adsorption and elution methods, which can be carried out according to the relevant literature.
