Perxidase(horseradish)；HRP；POD CAS 9003-99-0
Item No.： E0040
Store at： -20℃
English name：Perxidase(horseradish)；HRP；POD
Other names: Horseradish peroxidase; Peroxidase
Grade：BR
Molecular weight：40000
RZ≥1.5
Enzyme activity：≥150units/mg（Purity value RZ=A403nm/A275nm）
Vitality definition：One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20℃
PH：7.2
Physicochemical properties : Brown freeze-dried powder, a glycoprotein formed by the combination of colorless enzyme protein and brown iron porphyrin. HRP is composed of multiple isozymes, and the isoelectric point is PH3 ~ 9. The optimum pH for enzyme catalysis is slightly different due to different hydrogen donors, but mostly around pH5. The enzyme is dissolved in water and ammonium sulfate solution with saturation below 58%. The maximum absorption spectra of the auxiliary group and enzyme protein of HRP are 403nm and 275nm respectively. Generally, the purity of the enzyme is expressed by the ratio RZ (German Reinheit zahl) of od403nm / od275nm. The smaller the RZ value, the more non enzymatic proteins.
Purpose: Biochemical research. As one of the main raw materials of enzyme labeled antibodies, it is widely used in the enzyme linked reaction of secondary antibodies. It is a diagnostic enzyme for the production of clinical diagnostic kits; The biochemical diagnostic kits for blood glucose, triglyceride and cholesterol prepared with the enzyme and the ability of enzyme labeled antibody in enzyme-linked immunosorbent assay.
