DNase II ；Deoxyribonuclease Ⅱ(porcine spleen)；Deoxyribonucleate 3′-oligonucleotido-hydrolase
Item No.： E0047
Store at： -20℃
English name：DNase II ；Deoxyribonuclease Ⅱ(porcine spleen)；Deoxyribonucleate 3′-oligonucleotido-hydrolase
Other names: deoxygenated cell nuclease; Oxyribonucleic acid-5 ˊ- Oligonucleotide hydrolase; Ribonuclease free; Deoxyribonucleic acid polymerase; Deoxyribonuclease (porcine pancreas); Deoxygenated cell nuclease (porcine pancreas); DNase.
Grade：BR
Vitality：＞1000u/mg
Vitality definition：One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25℃； [Mg2+] = 0.83 mM
Loss on drying: ＜ 5.0%
Ignition residue: ＜ 1.0%
Physicochemical properties: White or off white lyophilized powder. It is a double stranded specific endonuclease, which can degrade double stranded DNA into 5 ˊ- Phosphoric acid and 3 ˊ- Oh terminal oligodeoxyribonucleotides. Single stranded DNA can also be degraded in the presence of monovalent manganese ions. Soluble in water, slightly soluble in 30% ethanol and acetone. Enzyme reaction: deoxyribonucleic acid → dinucleotide 5 ′ phosphate + oligonucleotide 5 ′ phosphate, the optimum pH is 7.8.。Light absorption coefficient (E1% 280) 11.1. The activator is divalent metal ions, and the maximum activation can be obtained from magnesium (Mg2 +) + calcium (Ca2 +). The inhibitors are EDTA and sodium dodecyl sulfate. 5mmol / L calcium (Ca2 +) can be added.
Purpose: Biochemical research. For molecular biology research. Various enzymes that degrade DNA. These enzymes hydrolyze the phosphate diester bond on the sugar phosphate main chain. There are two kinds of enzymes: exonuclease and endonuclease. There are many kinds of DNAase, some are single chain specific, some are double chain specific, or sequence specific (such as restriction endonuclease). Restriction endonuclease has the ability to recognize specific base sequences, which has been used in gene manipulation technology to construct artificial clones. This product is only for scientific research.
