Insulin Human recombinant
Item No.： P0029
Store at： -20℃
CAS：11061-68-0
Molecular formula: c257h383n65o77s6
Molecular weight: 5808
Grade: br
Potency: 15~31u/mg
Physicochemical Properties: white or off white crystalline powder. Insulin is produced by islets of Langerhans β A polypeptide hormone secreted by cells. Insulin plays an important role in many cell activities, such as promoting sugar and amino acid transport, improving anabolism, reducing catabolism, stimulating cell growth, and so on. Insulin is a common additive in serum-free mammalian cell culture medium. Recombinant human insulin is a polypeptide composed of 51 amino acid residues produced by recombinant technology. It is almost insoluble in water, ethanol and ether, and soluble in dilute hydrochloric acid and dilute sodium hydroxide solutions
Purpose: biochemical research. cell culture
Storage: -20 ° C
instructions:
The concentration of insulin in serum-free medium is generally 5~20mg/l. For different cell lines, the most appropriate concentration needs to be selected.
Recombinant human insulin was supplied in powder form and cryopreserved below -18 ℃. Dissolve it before use. Generally, hydrochloric acid solution (10mm) is used to make a stock solution containing 1~2mg insulin per 1ml, and then sterilize and filter it. After subpackaging, the filtrate can be stored at -20 ℃ for 6 months. Repeated freezing and thawing should be avoided.
