α-Amylase(Bacilus subtilis)；1,4-α-D-Glucan-glucanohydrolase CAS 9000-90-2
Item No.： E0003
Store at： 2～8°C
English Name: α- Amylase(Bacilus subtilis)； 1,4- α- D-Glucan-glucanohydrolase
Other names: medium temperature amylase; Liquefied amylase; Liquefying enzyme; α- 1,4 dextrinase; Bacterial amylase; α- Starch enzyme; Dextrinase
Grade: BR
Vitality: ≥ 4000U / g or 50U / mg for protein
Definition of activity: 1 ml of enzyme solution liquefied 1 g of soluble starch in 1 hour at 60 ℃ and pH 6.0 is an enzyme activity unit, expressed in U / ml
Moisture: ≤ 8%
Thermal stability: it is relatively stable below 60 ℃, and the optimum action temperature is 60 ~ 70 ℃, which is suitable for the liquefaction process up to 90 ℃
PH stability: it is relatively stable at pH 6.0 ~ 7.0, the optimum pH is 6.0, and the inactivation below pH 5.0 is serious
Effect of calcium ion concentration on enzyme activity: calcium ion can improve the stability of enzyme activity. Without calcium ion, the enzyme activity is completely lost
Physicochemical properties: yellowish brown or off white powder. Extracted from Bacillus subtilis and dissolved in water. At medium temperature, the enzyme can rapidly hydrolyze the refined starch molecules α- 1,4 glucoside bond, which arbitrarily cuts off starch molecules from the inside. Short bond dextrins with different lengths and a small amount of low molecular sugars, amylose and amylopectin are decomposed in an irregular form, so that the viscosity of starch slurry decreases rapidly (i.e. liquefaction, also known as liquidase). When the storage temperature is not more than 25 ℃, the enzyme activity shall not be less than 90% of the marked enzyme activity within half a year; In order to improve the stability of enzyme activity, the calcium concentration should not be less than 50-70ppm; Metal ions such as iron, copper, mercury and zinc and a surfactant have a certain effect on enzyme activity. When the liquefaction temperature is low or the material concentration is too high, the amount of enzyme must be increased appropriately; When the pH of the material is greater than 8.0 or lower than 5.0, the enzyme activity will be lost.
Purpose: biochemical research. It can liquefy starch rapidly to produce low molecular weight.
